Part:BBa_K208005:Experience

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Applications of BBa_K208005

Done by iGEM11 Potsdam_Bioware

The TorA signal sequence was used in combination with the beta lactamase (BBa_I757010) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase. When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C, which indicates a high export rate via the TAT pathway.

User Reviews

UNIQe72d5db0fa4f4c25-partinfo-00000000-QINU UNIQe72d5db0fa4f4c25-partinfo-00000001-QINU